Ultrasensitive Detection of Unstained Proteins in Acrylamide Gels by Native UV Fluorescence
نویسندگان
چکیده
منابع مشابه
Ultrasensitive detection of unstained proteins in acrylamide gels by native UV fluorescence.
Visualization of proteins inside acrylamide and other gels usually relies on different staining methods. To omit the protein-staining procedure, we visualized unstained proteins inside acrylamide gels by laser excitation with ultraviolet (UV) light (280 nm, 35 mJ/cm2) and directly detected native UV fluorescence. In one-dimensional gels, a detection limit as low as 1 ng for bovine serum albumin...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2003
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac020517o